Journal: iScience

Article Title: Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment

doi: 10.1016/j.isci.2024.108992

Figure Lengend Snippet:

Article Snippet: EC 50 /IC 50 values were calculated using a web calculator ( www.aatbio.com/tools/ic50-calculator ).

Techniques: Recombinant, Modification, Software, Control, Microscopy, Imaging, Flow Cytometry, Transmission Assay

Journal: bioRxiv

Article Title: Identification of SARS-CoV-2 Antiviral Compounds by Screening for Small Molecule Inhibitors of the nsp14 RNA Cap Methyltransferase

doi: 10.1101/2021.04.07.438810

Figure Lengend Snippet: a) Representative wells showing VERO E6 cells stained for DNA using DRAQ7, and viral nucleoprotein using Alexa 488 conjugated anti-nucleocapsid antibody (see methods). Top panel: Cells with no virus added. Lower panel: Cells with virus added to a MOI of 0.5 PFU/cell. b) After seeding cells, media was washed and replaced with fresh media containing compounds at indicated concentrations, followed by infection with SARS-CoV-2. Wells shown are representative of three biological repeats. DNA is visualised through staining by DRAQ7, and virus is visualised through staining for viral nucleoprotein (see methods). c) Quantification of viral and cell area through fluorescence microscopy of DNA staining by DRAQ7 and viral nucleoprotein (see methods). DNA and viral area measurements were initially normalised to the vehicle control. Values were then plotted as a percentage of the maximum normalised measurement. IC 50 values are given for each compound in the respective panel. Points represent mean values; error bars give standard deviation over three biological repeats. Error bars are not given if the error is smaller than the size of the point.

Article Snippet: Inauhzin demonstrated similar IC 50 values in the presence of remdesivir (Inauhzin EC 50 +Rem = 11.25 μM) ( ).

Techniques: Staining, Infection, Fluorescence, Microscopy, Standard Deviation

Journal: bioRxiv

Article Title: Identification of SARS-CoV-2 Antiviral Compounds by Screening for Small Molecule Inhibitors of the nsp14 RNA Cap Methyltransferase

doi: 10.1101/2021.04.07.438810

Figure Lengend Snippet: a) Representative wells showing cells after no infection, or infection with remdesivir or vehicle control. DNA is visualised through staining by DRAQ7, and virus is visualised through staining for viral nucleoprotein (see methods). It is known that < 1 μM remdesivir is ineffective in VERO E6 Cells (25). b) After seeding cells, media was washed and replaced with fresh media containing compounds at indicated concentrations, followed by infection with SARS-CoV-2. In addition, 0.5 μM remdesivir was added to all wells. Wells shown are representative of three biological repeats. DNA is visualised through staining by DRAQ7, and virus is visualised through staining for viral nucleoprotein (see methods). c) Quantification of viral and cell area through fluorescence microscopy of DNA staining by DRAQ7 and viral nucleoprotein (see methods). DNA and viral area measurements were initially normalised to the vehicle control. Values were then plotted as a percentage of the maximum normalised measurement. IC 50 values are given for each compound in the presence of remdesivir. Points represent mean values; error bars give standard deviation over three biological repeats. Error bars are not given if the error is smaller than the size of the point.

Article Snippet: Inauhzin demonstrated similar IC 50 values in the presence of remdesivir (Inauhzin EC 50 +Rem = 11.25 μM) ( ).

Techniques: Infection, Staining, Fluorescence, Microscopy, Standard Deviation